High sensitivity automated sequence determination of polypeptides.
نویسندگان
چکیده
منابع مشابه
High sensitivity automated sequence determination of polypeptides.
We report the development of a high sensitivity Edman method for use in the automated protein sequenator. With the use of a radioactive coupling reagent, [35S]phenylisothiocyanate, and minor modifications in the sequenator program, sequence analyses have been performed on nanomole quantities of protein. The radioactive phenylthiohydantoin derivatives produced in the degradation are identified a...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)41443-9